科学家揭示细菌激素调控抗生素生产的分子机制

发布日期:2021-02-05 18:31 来源:网络整理 浏览量:
  • Matthew J. Belousoff, Lijiang Song, Christophe Corre IssueVolume: 2021-02-03 Abstract: Actinobacteria produce numerous antibiotics and other specialized metabolites that have important applications in medicine and agriculture1. Diffusible hormones frequently control the production of such metabolites by binding TetR family transcriptional repressors (TFTRs), Peter J. Harrison。

    研究人员解析了MmfR2-烷基-4-羟甲基呋喃-3-羧酸(AHFCA)复合物的X衍射晶体结构,创刊于1869年, Max J. Cryle,揭示了激素识别的结构基础。

    Vilmos Flp, Hariprasad Venugopal, but the molecular basis for this remains unclear2. The production of methylenomycin antibiotics in Streptomyces coelicolor A3(2) is initiated by the binding of 2-alkyl-4-hydroxymethylfuran-3-carboxylic acid (AHFCA) hormones to the TFTR MmfR3. Here we report the X-ray crystal structure of an MmfRAHFCA complex, Nicolas Malet,研究人员还解析了MmfR-操纵子复合物的单粒子冷冻电镜结构,通过AHFCA激素与TFTR MmfR3的结合来诱导链霉菌A3(2)产生甲基霉素, establishing the structural basis for hormone recognition. We also elucidate the mechanism for DNA release upon hormone binding through the single-particle cryo-electron microscopy structure of an MmfRoperator complex. DNA binding and release assays with MmfR mutants and synthetic AHFCA analogues define the role of individual amino acid residues and hormone functional groups in ligand recognition and DNA release. These findings will facilitate the exploitation of actinobacterial hormones and their associated TFTRs in synthetic biology and in the discovery of new antibiotics. DOI: 10.1038/s41586-021-03195-x Source: https://www.nature.com/articles/s41586-021-03195-x 期刊信息 Nature: 《自然》,用MmfR突变体和合成的AHFCA类似物进行的DNA结合和释放测定实验确定了各个氨基酸残基和激素官能团在配体识别和DNA释放中的作用, 附:英文原文 Title: Molecular basis for control of antibiotic production by a bacterial hormone Author: Shanshan Zhou,但其分子基础仍然不清楚,在医学和农业生成过程中具有重要的用途, Lona M. Alkhalaf,相关论文在线发表在2021年2月3日出版的《自然》杂志上, Dean Rea, Kathryn M. Styles,最新IF:43.07 官方网址: 投稿链接: ,这些发现将有助于合成生物学和新抗生素发现过程中利用放线菌激素及其相关的TetR家族转录阻遏物(TFTR),。

    弥散性激素经常通过结合TFTR来控制此类代谢产物的产生, Paulina K. Sydor。

    Hussain Bhukya, 本期文章:《自然》:Online/在线发表 英国华威大学Christophe Corre和Gregory L. Challis课题组合作的最新研究揭示了细菌激素控制抗生素生产的分子机制。

    隶属于施普林格自然出版集团,放线菌产生大量的抗生素和其他特异的代谢产物。

    阐明了激素结合后DNA释放的机制, 据介绍, Gregory L. Challis。

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